Automated Synthesis of Peptides

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منابع مشابه

Automated synthesis of backbone protected peptides†

Solid phase peptide synthesis (SPPS) is a powerful technology for the chemical synthesis of peptides and small proteins. However, access to many targets is often complicated and sometimes precluded by the occurrence of so-called difficult sequences. When encountered during SPPS difficult sequences are associated with a collapse of the swollen resin volume, incomplete acylation and in the case o...

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Automated synthesis of backbone protected peptides† †Electronic supplementary information (ESI) available: Synthesis and characterization of peptides, experimental conditions. See DOI: 10.1039/c4cc03065f Click here for additional data file.

The synthesis of peptides rich in aggregation prone sequences can be improved with backbone protection. We report the automated introduction of backbone protection to a peptide. This new method was applied in a fully-automated synthesis, giving improved handling, quality and yield of several challenging target sequences.

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Synthesis of cystine-rich peptides

Abbreviations used: Acm, acetamidomethyl; Boc, tert-butoxycarbonyl; Bzl, benzyl; Bzl(4-Me), 4-methylbenzyl; DPDS, di(2-pyridyl)disulfide; DMSO, dimethylsulfoxide; Fmoc, 9-fluorenylmethoxycarbonyl; Mob, 4-methoxybenzyl; Npys, 3-nitro-2-pyridylsulfanyl; Pys, 2-pyridylsulfanyl; StBu, tert-butylsulfanyl; sec, selenocysteine; tBu, tert-butyl; TFA, trifluoroacetic acid; TfOH, trifluoromethanesulfonic...

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ژورنال

عنوان ژورنال: Kobunshi

سال: 1967

ISSN: 0454-1138,2185-9825

DOI: 10.1295/kobunshi.16.9_790